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How to get the extinction coefficient?

jh
jing he Jul 22, 2024
protocol Protocol: Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass

I have now measured the absorbance value and am ready to calculate the ABSL content result, how do I get the extinction coefficient I want, thank you very much!
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Charles T. Anderson Author Answered Jul 31, 2024

Department of Biology, The Pennsylvania State University, University Park, USA

Hi Jing, the extinction coefficient depends on the plant species for which you are measuring ABSL, but these values have been determined for many common species - see Table 1 and this reference: Johnson, D. B., Moore, W. E., and Zank, L. C. (1961). The spectrophotometric determination of lignin in small wood samples. Tappi, 44, 793-798.

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