Can mice be injected with suspension by intraperitoneal injection?小鼠腹腔注射可以注射混悬液吗?

佳丽 邵
May 24, 2024
protocol Protocol: Intraperitoneal Injection of Neonatal Mice

Can I use suspension for intraperitoneal injection in mice? In order to carry out the intraperitoneal injection experiment of baicalein, a PBS solution containing 10% DMSO was used to dissolve it, but the baicalein was not completely dissolved, and the liquid diagram is as follows. Can img Read more Read less more less

answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Amanda M Pocratsky Author Answered May 28, 2024

University College London London

Hi there!

It is generally not advisable to inject agents that have precipitated out of solution as this alters the concentration/dosage you are administering. I suggest you explore various methods to get your compound of interest fully dissolved. E.g. referring to the vendor MDS product info about solubility limits and reconstitution protocols, gradual heating, adjusting pH, using other vehicles, etc. All of this will need to be empirically tested until you develop a method that works consistently. Best of luck, and hope you find the neonatal injection protocol useful once you get to that stage!


All the best

Amanda

1 helpful
0 unhelpful
comment 3 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer