hello! I had some questions during my experiments similar with you guys.

BAPE HYUN
BAPE HYUN
May 13, 2024
protocol Protocol: Influenza Virus-cell Fusion Inhibition Assay
  • First of all, like CV-1 cells, Can I use this protocol which other cells can make MGC(Multinucleated Giant Cell) form in normal conditions?
  • Second, when fusion buffer inoculates each samples, is it possible time point may up to 15 minutes? because I used different influe Read more Read less more less
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Mayo Yasugi Author Answered May 31, 2024

Osaka Prefecture University

>First of all, like CV-1 cells, Can I use this protocol which other cells can make MGC(Multinucleated Giant Cell) form in normal conditions?


I have no idea. But if the cell line forms MGC and is infected with the virus, it would be available to the fusion assay.


>Second, when fusion buffer inoculates each samples, is it possible time point may up to 15 minutes? because I used different influenza virus genus and I think 2 minutes in incubator as you said is too short for my experiments.


I'm not sure. The buffer pH is 5.5. If your cells are not damaged by the buffer, you can extend the incubation time.


>Last question, in protocol, monoclonal antibody might be major target of HA? because HA mediates cell-cell fusion. Can this option also alternative for using the HA or HEF monoclonal antibody?


This assay is for the functional assessment of anti-HA/HEF MAb whether the MAb inhibits the fusion step.

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