Determination of protein synthesis in cells carrying puromycin resistance genes

光樑 陈
Mar 29, 2024
protocol Protocol: SUrface SEnsing of Translation (SUnSET), a Method Based on Western Blot Assessing Protein Synthesis Rates in vitro

Dear author, hello. May I ask if this method can be used to detect protein synthesis in cells transfected with the puromycin resistance gene lentivirus

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Cedric Duret Author Answered Apr 23, 2024

CRCL Lyon

I think yes. We did with stably transfected cells with puromycin resistance plasmids.

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