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Tomas Zavrel Author Answered Feb 17, 2024

Department of Adaptive Biotechnologies, Global Change Research Institute CAS, Czech Republic

Dear William,

We have not tested this protocol for plant cells. However, the (poly)saccharides hydrolysis by the concentrated sulfuric acid and chromophore formation in the presence of phenol (Procedure F) should work with identical efficiency in plants as in algae or cyanobacteria. Among the more than 60K works that cite the original paper of DuBios et al., 1956, there are many works that use the phenol-sulfuric acid method for plant material.

Also, the concentrated KOH (used for the hydrolysis of free sugars, Procedure D2-D5) penetrates easily into plant cells, which results in a highly efficient reduction of free sugars in plants as well as in cyanobacteria or algae.


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