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How to prepare 10 mM sodium citrate buffer?

Bhavs Vb
Bhavs Vb Dec 12, 2023
protocol Protocol: Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization

Hello sir,

I'd like to know the pH of the 1 Lit 10mM sodium citrate buffer that you prepared ?


Suppose I want to prepare 0.1M sodium citrate buffer of pH 3.0, I observed that most people use citric acid monohydrate (as weak acid) and&nbs Read more Read less more less

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Guang-Yuh Jauh Author Answered Dec 14, 2023

Institute of Plant and Microbial Biology, Academia Sinica, Taiwan

Dear colleague. Hello. We followed the recipe suggested by Cold Spring Harbor at: https://cshprotocols.cshlp.org/content/2009/12/pdb.rec12040.


Briefly, take 2.94 g of Trisodium Citrate (Dihydrate) (C6H8O7.2H2O.3Na) and carefully dissolve in 1L of distilled water. Please adjust the pH to 6.0 with 1M HCl. You may store the buffer at 4 Celsius for up to six months.


Best wishes,


Pablo

References

1.
https://cshprotocols.cshlp.org/content/2009/12/pdb.rec12040
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