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Why The YTK12 strain and YTK12 carrying the empty pSUC2 vector can grow on YPRAA medium?

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keyao ZHANG Nov 11, 2023
protocol Protocol: Functional Evaluation of the Signal Peptides of Secreted Proteins
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Weixiao Yin Author Answered Nov 22, 2023

Huazhong Agricultural University

Actually, the YTK12 strains with or without pSUC2 vector can grow on YPRAA, but the yeasts grow slowly compared with positive control for the first few days. Thus, you have to observe colonies timely.

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