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what is the template need to be used in this protocla and alse the primer used?

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Atheer Althobaiti Oct 3, 2023
protocol Protocol: Primer Extension Analysis Using AMV Reverse Transcriptase
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Harald Putzer Author Answered Oct 21, 2023

CNRS FRE3630, Institut de Biologie Physico-Chimique, France

You normaly use total RNA and a primer that is within about 50 to 150 nts from the 5‘ end you want to Identity. This allows to Identity the band on a sequencing gel with high résolution.

Good luck

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