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1, Anaesthetics cause blood flow to slow down, so is the measured blood flow still meaningful? 2, How do you locate the same area for blood flow shots

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dan hua hu Aug 7, 2023
protocol Protocol: Dynamic Analysis of Zebrafish Blood Flow by High-content Imaging System

1, Anaesthetics cause blood flow to slow down, so is the measured blood flow still meaningful?


2, How do you find the same blood flow shot area without swinging the fish in every hole


3, How long is the shot?
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毅 王 Answered Aug 14, 2023

Zhejiang University

  1. Our study (and many others) find 0.016% tricane anesthesia within 30-45 min usually does not influence the blood flow obviously. The inclusion of control group also helps to differentiate the impact of anesthetics and target drugs on the blood flow.
  2. We manually select a similar region (basing on the somite number, or other anatomical markers) for the analysis.
  3. 1-2 seconds.
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