Should we consider the buffer fir the Kdo assay?

protocol Protocol: Determination of Keto-deoxy-d-manno-8-octanoic acid (KDO) from Lipopolysaccharide of Escherichia coli

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So I'm isolating gram-negative bacteria vesicles and suspending them in a suitable buffer, such as PBS. Should I use 50 microliters of the vesicular solution suspended in the buffer to quantify Kdo content, or should the vesicular solution be suspended in MilliQ Read more Read less more less

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