After being induced with L929 supernatant for 6 days and identified to be macrophages by flow cytometry, does the medium containing L929 supernatant s

L929 cell-conditioned media is commonly used to differentiate bone marrow-derived monocytes (BMDMs) into macrophages in vitro. The conditioned media from L929 cells contains colony-stimulating factors that promote the differentiation of monocytes into macrophages. The inducted have already undergone cell differentiation and therefore you don't need to add additional L929 cell-conditioned media in the following experiments.

After your explanation, I understand. Thank you very much。

Wen Deng