Thanks for suggesting this easy and efficient way of isolating colonies. However, the tissue-tek isn't sterile. Will the cell culture get contaminate?

SW
Sandra Wong
May 18, 2023
protocol Protocol: A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Varun Kumar Author Answered May 19, 2023

University Hospital Heidelberg Heidelberg

Hi, it's supplied as a sterile liquid and one must open a new bottle under a sterile hood. further, in our protocol, Tissue tek just surround the cloning cylinders and it never come in contact with cells in the cylinder.


If you take care of other aseptic rules for cell culture, Tissue tek will not be a source of contamination.


After following this method, please don't forget to cite us.


All the best


0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer