Varun Kumar Author Answered May 19, 2023
University Hospital Heidelberg Heidelberg
Hi, it's supplied as a sterile liquid and one must open a new bottle under a sterile hood. further, in our protocol, Tissue tek just surround the cloning cylinders and it never come in contact with cells in the cylinder.
If you take care of other aseptic rules for cell culture, Tissue tek will not be a source of contamination.
After following this method, please don't forget to cite us.
All the best
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