After step 7 and before step 9, do I need to re-homogenize the sample and mix the pellet before observing in the spectrophotometer?

Donald Olsen
Donald Olsen
May 18, 2023
protocol Protocol: Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
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Tomas Zavrel Author Answered May 18, 2023

Department of Adaptive Biotechnologies, Global Change Research Institute CAS, Czech Republic

Dear Donald, it's quite the opposite. You should be careful not to disturb the pellet after step 7. The presence of debris in the path of the light beam of the spectrophotometer causes light scattering, which reduces the quality of the measurement. The absorbance at 720 nm (A720) in equations of step 9 is included exactly to correct for such scattering. However, it is always better to measure clean solvents with dissolved pigments only.

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