Whether the present technology that you apply for mammalian cells be used in microalgae for gene editing?

Prime editing has been shown to work in many different organisms- from E. coli (https://doi.org/10.1038/s41467-021-25541-3) to in vivo in mice (https://doi.org/10.1038/s41587-023-01758-z). Delivery of the prime editing components based on earlier techniques used to deliver Cas9 and an sgRNA (reviewed in https://www.frontiersin.org/articles/10.3389/fbioe.2020.00914/full) could enable prime editing in this organism, but optimization of the pegRNA and prime editor protein expression may be required.