I work on Marine microalgae. They grow in seawater. I want to know how the gene editing experiment can be carried out in microalgae that grow in seawater. Whether the seawater medium affect the experiment? Is there is gene delivery method specific to microalgae?
Jordan Doman Answered May 13, 2023
Prime editing has been shown to work in many different organisms- from E. coli (https://doi.org/10.1038/s41467-021-25541-3) to in vivo in mice (https://doi.org/10.1038/s41587-023-01758-z). Delivery of the prime editing components based on earlier techniques used to deliver Cas9 and an sgRNA (reviewed in https://www.frontiersin.org/articles/10.3389/fbioe.2020.00914/full) could enable prime editing in this organism, but optimization of the pegRNA and prime editor protein expression may be required.
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