How to access synthesized epegRNA?

When we want to use an epegRNA that is longer than the limit of RNA that companies can synthesize, we do remove the epegRNA motif to help decrease the length. Members of our lab have been able to obtain robust editing without the use of epegRNAs- see Chen et al 2021. As the synthesis capabilities of IDT and others expand, we hope that more epegRNA lengths will become accessible! We typically do not use in vitro transcription to generate epegRNAs because, as you mentioned, the lack of chemical modifications does decrease stability.