适用于转录因子类蛋白提取吗

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lin xiao di
May 4, 2023
protocol Protocol: Total Protein Extraction from Rice
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Wen-Tao Wang Answered May 19, 2023

Huazhong agricultural university

Transcription factors are mostly located in the nucleus and belong to regulatory proteins, with relatively low abundance. So this method is not recommended , for transcriptionla factors , It is recommended to perform nuclear extraction followed by auxiliary lysis before WB (please refer to the method of nuclear protein extraction)

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