How can we prepare the DSS-containing food for 3rd instar larvae, and when should we transfer them onto the food?

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Tadao Usui
Mar 27, 2023
protocol Protocol: Smurf Assay to Detect Drosophila Intestine Leakage

Please tell us the exact way to prepare the DSS-containing food for larvae. We can just add DSS-solution to the standard corn-meal fly food or have to make a special food? And also, when should we transfer the larvae to the food? The first instar stage, or the early third instar stage?

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kyan young Answered Apr 11, 2023

中科院

1.Thoroughly mix the DSS solution with normal fly food by the ratio of 1ml DSS solution /4g food.

2.Collect 3rd instar feeding larvae and transfer to the food.

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