What is the storage condition for the isolated complexes and does this condition maintain it native form ?

Hi,

We flash-freeze them in liquid nitrogen and then store them at -80C. The storage buffer is 20mM Hepes-KOH pH 7.4, 20mM sodium chloride, 50mM potassium acetate, 2mM magnesium chloride, 0.1% Tween-20, 10% glycerol, 1mM DTT. The glycerol acts as a cryo-preservative and under these conditions the nuclear pore complexes maintain their native state after isolation for several weeks.