What is the optimal condition to CoIP an endogenous tagged protein without losing any of its interaction partners?

Hi,

I am sorry to say that there is no magic recipe for that, it is completely case-dependent and it usually requires to invest time and effort on the optimization of the affinity purification conditions. We will try to show some general guidelines for the selection of proper conditions that could help maximize the recovery of the tagged protein and its interactome using as an example the nuclear pore complex.