Why doesn't the strip run in a straight line in a western blot?

1. It is very likely that your gel is uneven or not solidified well, the upper gel and the lower gel solution should be gently shaken to mix evenly, and after adding TEMED, you can use the tip to suck and mix well and then inject into the glass plate. You can extend the sealing time appropriately, confirm that the sealing is complete and then add the lower layer of gel, once of my sealed, the middle part of the gel did not solidify well, and the solution flowed out after adding the lower gel. If there is enough time, all gel time can be extended to allow the gel to solidify sufficiently, especially when the room temperature is low.  

2. It may be that the gel configuration reagent is unqualified, it is recommended to check the reagents of each component and reconfigure those reagents that have been stored for a long time.