hi there
why ascorbate is added and what's the volume of ascorbate?Thanks
Praveen Kumar Author Answered Jan 26, 2023
CCS Haryana Agricultural University Hisar
Hi there,
Ascorbate is added because the Ascorbate peroxidase enzyme scavenges reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) to water using reduced ascorbate as the electron donor.
For your query about the volume of Ascorbate to be used is 0.05 to 0.1 ml. Moreover, you have to standardize the protocol with your sample by heat and trial method with different volumes of ascorbate by keeping the total volume of the reaction mixture constant. Adjust the variable volume of ascorbate by varying the volume of buffer in the total reaction mixture.
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