The function of ascorbate

YAN AI
YAN AI
Jan 25, 2023
protocol Protocol: Measurement of Ascorbate Peroxidase Activity in Sorghum

hi there

why ascorbate is added and what's the volume of ascorbate?Thanks

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Praveen Kumar Author Answered Jan 26, 2023

CCS Haryana Agricultural University Hisar

Hi there,

Ascorbate is added because the Ascorbate peroxidase enzyme scavenges reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) to water using reduced ascorbate as the electron donor.

For your query about the volume of Ascorbate to be used is 0.05 to 0.1 ml. Moreover, you have to standardize the protocol with your sample by heat and trial method with different volumes of ascorbate by keeping the total volume of the reaction mixture constant. Adjust the variable volume of ascorbate by varying the volume of buffer in the total reaction mixture.

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