What is the final concentration for Triton X-100 in Cell lysis and capturing buffer? 1% or 0.01%

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Hao Ding
Jan 24, 2023
protocol Protocol: Yeast Single-cell RNA-seq, Cell by Cell and Step by Step

The protocol uses 0.05 microliter of 1% Triton X-100 in a total of 5 microliter buffer, which gives a final concentration of 0.01%. My experience with yeast lysis buffer usually has Triton X-100 at 1% final concentration, I wonder whether you could confirm that the final concentration Read more Read less more less

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