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Why use Glucanex(Sigma，L1412).?

小南侯 Jan 9, 2023

Protocol: Sclerotinia sclerotiorum Protoplast Preparation and Transformation

Glucanex(Sigma，L1412)’s production has already stopped. So maybe this protocol can not be repeat now. So, do you have other suggest enzymes for this?

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Chi Lan Author Answered Apr 17, 2023

Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095

Maybe you can try Lysing Enzymes from Aspergillus sp. (L3768-1G, Sigma-Aldrich), which contains pectinase and cellulase activities, being used to lyse mycelium cell wall. Of course, the following reference can help you obtain mutant of Sclerotinia sclerotiorum quickly.

References

A Protoplast Transformation System for Gene Deletion and Complementation in Sclerotinia sclerotiorum ( https://doi.org/10.1111/jph.12137)

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