Question about the molarity of the buffer and concentrations

protocol Protocol: Fluorometric Estimation of Glutathione in Cultured Microglial Cell Lysate

Hello dear Vikas Singh!


Very nice protocol but I still have some small questions:


Is there a reason the amount of EDTA disodium is 0.16 g instead of 0.186 (0.005 M in 100 ml) and K2HPO4 0.85 instead of 0.87 (closer to 0.1 M)?


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Debabrata Debabrata Ghosh Answered Dec 9, 2022

CSIR-Indian Institute of Toxicology research

Hi Leonhard


For EDTA-disodium dihydrate 0.186 g is correct and K2HPO4, 0.87 gm is correct.


Thank you for pointing out the mistake.


For OPT solution, it is easier to weigh 10 mg instead of 1 mg. Therefore, we prefer to make 10 mg/ml stock rather than 1 mg/ml.


I hope this will answer your queries.


Thank you

Vikas

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