Is there an error in my colony PCR?

Yes, normally, when setting up a positive control, we will use a plasmid containing the fragment of DNA to be joined, in order to determine the location of the DNA and facilitate the search for the correct clone, so your DNA bands should appear in the swimlane of the positive control. The negative control is mainly to prevent the use of reagents or DDH2O contamination, resulting in false positive results, which should normally be without bands. So, if you don't reverse the positive and negative controls, then you have problems with both controls in this experiment. For positive controls, the plasmid you are using may be degraded or the plasmid concentration and volume may be added. In addition, the reagent or DDH2O you use is likely to be contaminated, especially DDH2O, it is recommended to use freshly sterilized unopened water, usually you can divide some into centrifuge tubes and keep them in -20 for backup.