
I found that some people add some sodium azide to the primary antibody dilution when experimenting, but I usually do not add this to the experiment, will this have any effect?
Amy Markovics Answered Nov 27, 2022
Midwestern University
Some people are used to adding 0.01%-0.05% sodium azide to the primary antibody dilution, sodium azide has a certain antiseptic effect, can prevent antibody deterioration, so that the use of antibodies is longer. High concentrations of sodium azide interfere with all coupling reactions involving amine groups, and it is easy to quench with HRP, affecting the appearance of protein bands, so it is not advisable to add too much sodium azide to the antibody dilution. In addition, when conducting experiments do not add sodium azide if it possible, even if you do not add it, the antibody can be reused 3-5 times.
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