Does a long blocking cause the background high in western blot?

After transfer, we need to block some sites on the PVDF membrane that do not bind to proteins to reduce the non-specific reaction of the membrane with the primary antibody, so this step is required. If these sites are not completely occluded, the background of the membrane will be high, which will also affect the strength of the signal. Blocking for too long can reduce the sensitivity of the membrane and sometimes even obscures the signal of the target protein, making the resulting protein bands weak.