What are the advantages of this flow cytometry based protocol over others, if any?

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Deblina Guha
Nov 24, 2022
webinar Webinar: Chromatin binding of RNA polymerase II and its phosphorylated forms through the cell cycle by flow cytometry

Can this method be useful for detection of any signaling molecular event in cancer cells?

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Helga Bjarnason Landsverk Answered Nov 29, 2022

Department of Radiation Biology, Institute for Cancer Research, Norway

Compared to other flow cytometry methods looking at chromatin binding proteins, this protocol has several advantages:


  • barcoding eliminates sample to sample variation during staining
  • precise determination of cell cycle location by EdU
  • optimized extraction and fixation conditions


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