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Is it possible to distinguish by this technique the RNAPII elongation rate over gene bodies?

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Noé Seija Nov 21, 2022
webinar Webinar: Chromatin binding of RNA polymerase II and its phosphorylated forms through the cell cycle by flow cytometry

I'm thinking to couple this technique with DRB release to measure transcription elongation speed.
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Helga Bjarnason Landsverk Answered Nov 29, 2022

Department of Radiation Biology, Institute for Cancer Research, Norway

No, you would need sequence specific information to measure elongation rate. The method rather answers the question is elongation affected? If yes, then pRNAPII S2 is likely reduced. However, elongation might be reduced with pRNAPII S2 levels high, such as e.g. occurs at early timepoints after UV.

References

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https://doi.org/10.1093/nar/gkac434
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