Why are there so many non-specific bands in my PCR results?

1. It may be that your primer specificity is weak, so it is easy to produce non-specific bands, or self-polymerization to form primer dimers, it is recommended to check the primer specificity, if necessary, replace the primer.  

2. The template is contaminated, mixed with other nucleic acids and primers are combine to produce non-specific bands.  

3. PCR reaction conditions are not suitable, annealing temperature is low or the number of cycles is large, the non-specific binding of primers and DNA templates in the reaction system, resulting in a large number of non-specific binding bands in the reaction results. If the non-specific bands are smaller than the gene of interest, the annealing temperature can be reduced and the number of cycles can be reduced, and if the non-specific bands are larger than the band of interest, the extension time can be shortened and the number of cycles can be reduced.