How to deal with the difference between blank and negative control of real-time quantitative PCR?

Hello!

There will be some differences between the blank control and NC group. Because the transfection of small RNA will certainly have a certain impact on the cell itself, and then affect the expression of some factors, which belongs to the influence of experimental operation. But in your experiment, the ratio of blank control group to negative control group was 0.6:1, is it before or after the reference? If there is no reference correction, then the comparison is meaningless. If it is after reference correction, then your blank group is different from the NC group in question. I'm guessing there might be something wrong with your NC clip.

Good luck!