Why is my RT-PCR amplification curve is jagged and the ct value is large?

 1. Discontinuities in the amplification curve are caused by low RNA purity or low gene expression. It can be recommended to increase the gradient of the template dilution multiple to see the optimization effect or re-prepare high-purity RNA to re-experiment  

2. It may be that the instrument was used for a long time, and the baseline needs to be adjusted. 

3. The entire reaction conditions of RT-PCR are not suitable or the primer design is improper, resulting in low amplification efficiency, it is recommended to confirm the amplification efficiency by standard curves.