
Recently I was preparing to perform qPCR to detect the expression of a gene, first of all, a semi-quantitative experiment was conducted to detect the specificity of the primers, and it turned out that the specificity of the primers was good but the primer dimers were relatively strong,
Read moreSharon Akanbi Answered Oct 9, 2022
University of Leeds
The most fundamental reason for the emergence of primer dimers is the complementary combination of bases between primers or at the 3' end of the primers themselves, in addition, low template amounts, large primer concentrations, short primer lengths, low annealing temperatures, and too many cycles can also lead to primer dimers. Therefore, the fundamental way to reduce the primer dimer is to redesign a more suitable primer, and at the same time, attention should be paid to the concentration ratio of the primer and the template in the PCR reaction solution, the annealing temperature, the number of cycles and other links.
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