How can I reduce the formation of primer dimmers in qPCR?

Jennifer N
Jennifer N
Oct 8, 2022

Recently I was preparing to perform qPCR to detect the expression of a gene, first of all, a semi-quantitative experiment was conducted to detect the specificity of the primers, and it turned out that the specificity of the primers was good but the primer dimers were relatively strong, Read more Read less more less

answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Sharon Akanbi Answered Oct 9, 2022

University of Leeds

The most fundamental reason for the emergence of primer dimers is the complementary combination of bases between primers or at the 3' end of the primers themselves, in addition, low template amounts, large primer concentrations, short primer lengths, low annealing temperatures, and too many cycles can also lead to primer dimers. Therefore, the fundamental way to reduce the primer dimer is to redesign a more suitable primer, and at the same time, attention should be paid to the concentration ratio of the primer and the template in the PCR reaction solution, the annealing temperature, the number of cycles and other links.

14 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer