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how do we determine the volume of FC reagent and sodium carbonate to be added to samples/standard?

NARMATHA GURUMOORTHY MMB212005
NARMATHA GURUMOORTHY MMB212005 Sep 19, 2022
protocol Protocol: 4.4. Folin–Ciocalteu Method to Measure Total Phenolic Contents

i am not sure on how to decide the vol of FC and sodium carbonate to add?
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