Is there any difference in centrifugal speed and time between serum and plasma preparation?

1. Serum separation: After the blood was obtained, let it stand in an EP tube for more than one hour at room temperature (or in a water bath at 37°C for 1 hour, and in a refrigerator at 4°C for 2 hours or overnight), centrifuge at 3000 rpm for 10 minutes, and the supernatant is serum. Can be stored at -80°C.
2. Plasma separation: Use a dried heparin tube or a heparin anticoagulant tube with a small amount of liquid to fully mix the obtained whole blood and anticoagulant, and the supernatant obtained after centrifugal force at 3000 rpm for 10 minutes is the plasma sample. It can be stored at 4°C for a few days, and it can be stored at -20°C or -80°C for a longer time, but if you want to measure the activity of lactate dehydrogenase, it must be measured immediately after blood sampling to obtain accurate results.

The serum and plasma depend on whether you add an anticoagulant like heparin. The centrifugal conditions are the same.

1. the preparation of serum: the blood can not be anticoagulant! Put the blood in a centrifuge tube or a vessel that can be centrifuged and put it in a static or 37℃ environment to promote its coagulation. After blood coagulation, centrifuge (usually at 3000rpm, 5 ~ 10min), the supernatant obtained is the serum, which can be carefully sucked out (be careful not to suck out cell components), and then divide and reserve.

2. Plasma preparation: add a certain proportion of anticoagulant (anticoagulant: blood = 1:9) to the blood container. The blood is added to a certain amount and then reversed and mixed. The supernatant obtained after centrifugation (centrifugation conditions are the same as above) is plasma. The primary user had better move the supernatant to another clean container, suction plasma with a capillary pipette with the liquid level gradually suction, avoid by all means can not suck up cell components.