What are the functions of SDS, SDS-PAGE,AB,AP and TEMED in SDS polyacrylamide gel electrophoresis?

SDS can destroy the disulfide bonds of proteins to denature proteins and alter protein structure, at the same time, a large negative charge is attached to the protein.

SDS -page is a prefabricated gel, that is a kind of gel that is pre-made in plastic panels. It can be used directly. 

AB is gel precursor

AP can catalyze monopropyl and bipropylene polymerization into polyacrylamide. TEMED can is a accelerator, accelerates AP catalysis. They are used to polymerize gels.

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SDS destroys the disulfide bond of the protein, denatures the protein, changes the structure of the protein, and attaches a large amount of negative charge to the protein. AB is the gel precursor. Ammonium persulfate (AP) was used as a catalyst and tetramethylene diamine (TEMED) as an accelerator. In the polymerization process, TEMED catalyzes ammonium persulfate to generate free radicals, which trigger the polymerization of acrylamide monomer and generate methyl-bisacrylamide cross-linking with acrylamide chain, thus forming a three-dimensional network structure.