How can I tell if a protein has formed a dimer?

It can be judged by the band size in the result, if the protein forms a dimer or multimer, there will be significant multiple relationships between the protein size in the western and the predicted value, so if the result has bands twice the predicted size, there are dimers present in the sample.

Hello, you can judge whether a protein is a monomer or a dimer by native electrophoresis. Native electrophoresis is Native-PAGE, which is different from normal SDS-PAGE without adding denaturing agents such as SDS and mercaptoethanol. Polyacrylamide gel electrophoresis was performed on proteins that remained active. According to the comparison between the molecular weight of the protein run out by Native-PAGE and the theoretical molecular weight, it can be judged whether it is a monomer or a dimer.