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Protein Expression

When inducing protein expression of E. coli, how to choose OD600, induction temperature, and concentration of IPTG?

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Yamini Jakubowski Aug 19, 2022

What conditions can be optimized when using IPTG to induce E. coli protein expression？
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David Shafi Answered Aug 21, 2022

California State University, Chico

OD600：0.6-1， induction temperature：28 ℃/37 ℃， the concentration of IPTG：0.5-1 mM

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