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RNA

Thank you for sharing your protocol! What is the reagent "VRC"? Ribonucleoside Vanadyl Complex ? Also, about how much RNA do you usually end with?

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Benjamin Chadwick Aug 13, 2022
protocol Protocol: Measurement of mRNA Decay in Mouse Embryonic Fibroblasts

For example, about how much RNA do you have after SSD1 IP?
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James Hose Answered Aug 23, 2022

Correct, Ribonucleoside Vanadyl Complex (Promega S1402S). We typically obtain 150-200ng RNA from the IP.


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