I would like to know more about how to grow my cells on the microscope slide. How do you keep the setup sterile, how do you provide the optimal growth conditions, and how long do you usually grow them before imaging?
Hi William, from my experience, I am using the Glass Bottom Dishes to grow cells for microscope imaging. Different types of glass bottom with different size, such as 24 well-plate or 35 mm glass dishes; coated or non-coated can be purchased from different suppliers. They are really great for cell culture and high resolution imaging.
They are working for both fixed cells and live cell imaging. For the live cell imaging, we have the incubator together with the microscope that controlling the growth conditions, such as temperature, CO2 and sterile. For different aim, I usually culture cells for 24hours or 48hours before imaging. Hope it answers you.
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