How we can see genes and how to check its functionality?

HI Divya Bhagat,

there might be several ways of visualizing gene expression - or mRNA levels -, one utilizes an in situ hybridization technique where a single-stranded and fluorescently labelled DNA probe hybridizes with your RNA of interest. That techniques enables quantification of both the amount (quantity) of the RNA and its localization within the cell. Here is a protocol explaining how you can apply this technique:

https://bio-protocol.org/e3070