How to use ImageJ to measure green fluorescence intensity?

ImageJ can be a very convenient software for processing image information. For fluorescence determination, you can 

1. Loading the image to ImageJ

2. Convert image format: RGB →8 bit

3. Choose a threshold in your channel

4. Set a measurement: Area (the Area of the selected Area); Mean (Mean gray value of the selected area of the box); IntDen (Integrated Density) (total fluorescence intensity in the selected area);

5. Analysis

The detailed protocol you can see in the following websites:

1. https://www.abbkine.com/analyze-the-fluorescence-intensity-of-cells-by-using-image-j/

2. https://theolb.readthedocs.io/en/latest/imaging/measuring-cell-fluorescence-using-imagej.html