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How to avoid band smiling in a slab gel electrophoresis. What is the reason of gel smiling?

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Chenchen Liu Jul 8, 2022

The experiment was carried out at room temperature around 25 oC.
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胡 Answered Jul 9, 2022

中国科学院大学

Smiling face bands appear in WB, which may be due to uneven cooling of gel and poor intermediate cooling; And the electrophoresis voltage is too high, which makes the temperature of the electrophoresis system high.

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