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What is the role of ethanol in Bradford reagent?

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建国 阮 Jul 2, 2022
protocol Protocol: Bradford Protein Assay

What is the role of ethanol in Bradford reagent?
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Georges Charbel Chalhoub Answered Jul 2, 2022

Queen Mary university London

Ethanol in Bradford acts as a reacting functional group since the OH at the end of the ethanol/methanol can be deprotonated( the H leaves the oxygen) in either slightly acidic(+H3O) or slightly basic conditions( -OH). And once the ethanol becomes deprotonated, another compound can be added to the carbanion.

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