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Sample Storage

How long can I keep my western blot samples at 4°C for, after adding loading dye, DTT and boiling for 5mins?

Billy Bednarek
Billy Bednarek Jun 21, 2022

I'm looking at phosphorylated proteins in triple negative breast cancer cells, so they are quite sensitive. I was wondering how long can I store my samples at 4°C after mixing them with loading dye and DTT and then boiling for 5mins?
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Jessica Biswas Answered Jun 24, 2022

University College London

The protein samples mixed with the loading dye and DTT can be stored at 4℃ for about a month. However, it is generally not recommended to store at 4℃. It is recommended to store at -20℃ or lower. Boil and centrifuge before each use.

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