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SiRNA

Dose of siRNA, type of medium and method to check ?

hn
huong ngo Jun 9, 2022
protocol Protocol: siRNA Transfection of Mouse Bone Marrow-derived Macrophages
  1. I would like to ask about the dose of siRNA the author used. How much ng/well of siRNA did you use for 24-well plate and 6-well plate?
  2. Did you add MCSF or GMCSF into your culture medium?
  3. How is the lockdown efficiency of siRNA negative control, positive control and Read more Read less more less
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Dan-Dan Ma Answered Jun 19, 2022

Peking University

For testing the efficiency of siRNA, you can either choose qPCR to test the target mRNA，or Western blot to test the protein level after transfection for 24-72 hours.

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