Cell lysis after IPTG induction?

Akira Karasawa
Akira Karasawa
May 24, 2022

I'm expressing a recombinant protein in e.coli BL21pLyss strain using pET expression system. After adding IPTG (final conc. 0.1, 0.3, and 1mM) at OD600 = 0.8 in TB medium, cells were lysed after a few hours of induction at 37C. Transformants without IPTG kept growing. Does someone have Read more Read less more less

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Augustin Alloncle Answered May 26, 2022

Prince of Songkla University

I understand what you're talking about, but what exactly are you asking? Do you mean that your cells are lysed after induction by adding IPTG?

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