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Flow cytometry
Apoptosis
Cell Biology

Why can't EDTA-containing trypsin be used when using Annexin V to detect apoptosis by flow cytometry?

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Parminder vijayan May 12, 2022

Adherent cells in flow cytometry apoptosis experiment, why can't EDTA-containing trypsin be used when using Annexin V to detect apoptosis by flow cytometry?
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Hyungjin Khaleque Answered May 13, 2022

Vanderbilt University Medical Center

EDTA will chelate Ca2+, thereby affecting the binding of Annexin V to PS.

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