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Protein Expression

When we can add IPTG to induce protein expression?

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Ez-zahra DARIF May 5, 2022

When using E. coli prokaryotic protein expression, when should I add IPTG to induce protein expression?
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Virginia Wehenkel Answered May 5, 2022

Addis Ababa University

IPTG should be added when cell growth reached the exponential phase (OD600=0.6~0.8).

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